Asterick is not connecting caller to agent after first call

Cheers for the reply, ambiorixg12.

Here’s the output before the first call is made:

8dca4d752649*CLI> sip show peers
Name/username             Host                                    Dyn Forcerport Comedia    ACL Port     Status      Description                      

test-agent/test-agent     <ip>                             D  Auto (No)  No             32287    Unmonitored                                  
twilio1                   <ip>                                Auto (No)  No             5060     Unmonitored                                  

twilio2                   <ip>                                 Auto (No)  No             5060     Unmonitored                                  
twilio3                   <ip>                                 Auto (No)  No             5060     Unmonitored                                  
twilio4                   <ip>                                 Auto (No)  No             5060     Unmonitored                                  
5 sip peers [Monitored: 0 online, 0 offline Unmonitored: 5 online, 0 offline]

It remains the same after the first call is complete and before the second, failing, call is made